|
KMID : 0613820110210040561
|
|
Journal of Life Science
2011 Volume.21 No. 4 p.561 ~ p.567
|
|
|
Anti-Inflammatory and Anti-Oxidative Activity of Methanol Extract from Terminalia chebula Retz., Lavandula spica L., and Dalbergia odorifera T. in RAW 264.7 Cells
|
|
Chae In-Gyeong
Yu Me-Hee
Kim Hyuk-Il
Lee In-Seon
|
|
|
Abstract
|
|
|
|
This study was performed to evaluate the anti-inflammatory and antioxidant activities of methanol extract from natural products. Cell viability was determined by MTT assay. The production of NO and TNF-α were measured by Griess assay and enzyme-linked immunosorbent assay (ELISA). In order to effectively screen for anti-inflammatory agents, we first examined the inhibitory effects of 24 natural products on the production of lipopolysaccharide (LPS)-induced nitric oxide (NO) in RAW 264.7 cells. Three extracts of Terminalia chebulaRetz., Lavandula spicaL., and Dalbergia odoriferaT. significantly inhibited NO production. The three extracts significantly decreased production of NO in a dose-dependent manner. Terminalia chebulaRetz. decreased TNF-α production. Antioxidative effects of the three extracts were measured based on polyphenol and flavonoid contents and DPPH radical scavenging activity assay. The three extracts showed high polyphenol contents as well as strong DPPH scavenging activities. In particular, Terminalia chebulaRetz. contained the highest polyphenol and flavonoid levels of 616 and 96 ㎍/㎎, respectively, compared to Lavandula spica L. and Dalbergia odoriferaT. As DPPH radical scavensing activities, RC50 values of Terminalia chebulaRetz. were 2.09 ㎍/㎖.
|
|
|
KEYWORD
|
|
|
Anti-inflammatory, anti-oxidantive, Dalbergia odoriferaT. Chen., Lavandula spica L., Terminalia chebula Retz
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|